Proteomic and functional genomic analysis of the host-pandemic virus interaction

Summary

Evolution has produced an arms race between viruses and the cells they infect. Studying this battle provides key insights into cell biology and immunology, as well as the viruses themselves. It may even lead to the development of novel therapeutics. The Matheson lab therefore focuses on two pandemic viruses with a major impact on human health: HIV and SARS-CoV-2. 

Project aims

Our aim is to use unbiased proteomic and CRISPR/Cas9-based genetic screens to quantify dysregulation of proteins in infected cells, discover host genes regulating viral pathogenesis, and understand the roles of these factors in normal cellular physiology. We are particularly interested in viral and cellular regulation of amino acid metabolism and protein biosynthesis. 

Depending on the preferences of the student, this project may focus on HIV or SARS-CoV-2, and explore either mechanisms of cell-to-cell spread, or metabolic pathways exploited by the viruses. In each case, the aims will be to: identify and validate key cellular targets; define mechanisms of viral regulation and/or dependence; and characterise their impact on viral infection and immunobiology. 

As well as opportunities to conduct proteomic and/or genetic screens, the project will provide training in a wide range of molecular and biochemical techniques. The Matheson lab is based in the new Cambridge Institute of Therapeutic Immunology & Infectious Disease (CITIID), including the largest academic Containment Level 3 (CL3) facility in the UK. The student will be supervised by an experienced postdoc in a friendly, supportive group.

Contact details

Dr Nicholas Matheson - njm25@cam.ac.uk

Opportunities

This project is open to applicants who want to do a:

• PhD